Studies on phospholipids. 8. Phospholipids in rat-liver mitochondria and microsomes

Exchange of phospholipids between liver mitochondria and microsomes in vitro.

A 10% rat liver homogenate in 0.25 M sucrose-10-3 M ethylenediaminetetraacetate was fractionated into mitochondria, microsomes, and a supematant fraction. Liver mitochondria derived from rats injected with leucine-3H and with phosphate-32P or with glycerolJ4C were incubated with nonlabeled microsomes in the presence of supernatant or of sucrose-EDTA. Labeled microsomes were similarly exposed to...

Studies on Phospholipids. 9. the Composition of Rat-liver Lecithins.

Rat-liver Mitochondria and Microsomes

Work by Marinetti, Erbland & Stotz (1958) on pig-heart-cell fractions and by Macfarlane, Gray & Wheeldon (1960) on rat-liver-cell fractions have indicated that the mitochondrial and microsomal phospholipids may differ significantly in composition. In view of the results obtained by Collins (1960) and by Collins & Shotlander (1961), showing the widespread occurrence of complex amino-phospholipid...

Synthesis of Glycerides and Phospholipids in Rat Liver

1. The effects on glycerolipid synthesis of a series of compounds including many drugs were investigated in cell-free preparations and slices of rat liver. 2. p-Chlorobenzoate, p-chlorophenoxyisobutyrate, halofenate, D-amphetamine, adrenaline, procaine and N-[2-(4-chloro-3-sulphamoylbenzoyloxy)ethyl]norfenfluramine had little inhibitory effect on any of the systems investigated. 3. Two amphiphi...

Physical Studies of Phospholipids

On heating pure, fully saturated 2,3-diacyl-DL-phosphatidyl-ethanolamines and 2,3-diacylphosphatidyl-cholines (lecithins) in water to the transition temperature at which large endothermic heat changes occur, they are observed, by light microscopy, to form myelin figures. This result is discussed in terms of the large difference in the transition temperature for "melting" of the hydrocarbon chai...